ONE-STEP NUCLEIC ACID EXTRACTION AND AMPLIFICATION ON AN INTEGRATED MICRODEVICE FOR EARLY DETECTION OF Cochlodinium polykrikoides
نویسندگان
چکیده
Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell – Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea – was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection.
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